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Bacterial culture and isolation techniques
This is a mind map about bacterial culture and isolation technology, including the classification of culture media, Preparation of culture medium, Artificial culture of bacteria, etc.
Edited at 2024-03-07 13:04:58- bacteria
This is a mind map about bacteria, and its main contents include: overview, morphology, types, structure, reproduction, distribution, application, and expansion. The summary is comprehensive and meticulous, suitable as review materials.
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Bacterial culture and isolation techniques
- bacteria
This is a mind map about bacteria, and its main contents include: overview, morphology, types, structure, reproduction, distribution, application, and expansion. The summary is comprehensive and meticulous, suitable as review materials.
- Plant asexual reproduction
This is a mind map about plant asexual reproduction, and its main contents include: concept, spore reproduction, vegetative reproduction, tissue culture, and buds. The summary is comprehensive and meticulous, suitable as review materials.
- Reproductive development of animals
This is a mind map about the reproductive development of animals, and its main contents include: insects, frogs, birds, sexual reproduction, and asexual reproduction. The summary is comprehensive and meticulous, suitable as review materials.
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- Outline
Bacterial culture and isolation techniques
medium
Definition: Mixed nutrients prepared by artificial methods and suitable for the growth and reproduction of microorganisms.
Main ingredients
Nutrients
protein
Meat dip
beef paste
carbohydrate
blood
Eggs and animal serum
Inorganic salt
growth factors
water
A good solvent, usually distilled water or ion exchange water without impurities
solidified substance
Agar (most suitable gelling substance), gelatin, egg white, serum
Solid or semi-solid media
inhibitor
It is a type of bacteria that can inhibit or reduce the growth of non-detectable bacteria. Substances conducive to the growth of detected bacteria
Select media
indicator
In order to observe and identify whether sugars, amino acids and other substances are decomposed and utilized, often Certain types of indicators are added to certain media
Acid-base indicators: phenol red, bromocresol green, bromothymol blue
Classification of culture media
Classified by use
basic medium
Contains essential nutrients required for bacterial growth
broth, plain agar plate
Nutrient medium
Add blood, serum, Growth factors and other special substances
blood agar plate
chocolate agar plate
Select media
Adding certain types of inhibitors to the culture medium, Inhibit the growth of non-target bacteria in specimens, selectively A medium that promotes the growth of the target bacteria
ss agar medium
identification medium
Add specific substrates and indicators to the culture medium, Observe the release of bacteria as they break down substrates Different products can be identified by different reactions of indicators
sugar fermentation tube
special culture medium
anaerobic medium
Bacterial L-type medium
According to physical properties
liquid culture medium
semi-solid medium
solid medium
Preparation of culture medium
allocate
Dissolve
Correct pH
Packaging
Sterilize
Quality inspection
save
Artificial culture of bacteria
Aseptic technique
Vaccination tools
inoculation loop, inoculation needle
Bacterial inoculation and isolation methods
Flat line drawing method
Zoning Lines
continuous line drawing
Agar plate slant inoculation method
liquid inoculation
puncture vaccination
coating inoculation method
pour inoculation