MindMap Gallery High School Biology-Enzyme Mind Map
This is a mind map about enzyme knowledge point ⑤, which is a compulsory course in high school biology. It introduces the nature of enzymes, the experience of exploring the nature of enzymes, the characteristics of enzymes, The role of enzymes, etc.
Edited at 2023-12-03 17:18:38El cáncer de pulmón es un tumor maligno que se origina en la mucosa bronquial o las glándulas de los pulmones. Es uno de los tumores malignos con mayor morbilidad y mortalidad y mayor amenaza para la salud y la vida humana.
La diabetes es una enfermedad crónica con hiperglucemia como signo principal. Es causada principalmente por una disminución en la secreción de insulina causada por una disfunción de las células de los islotes pancreáticos, o porque el cuerpo es insensible a la acción de la insulina (es decir, resistencia a la insulina), o ambas cosas. la glucosa en la sangre es ineficaz para ser utilizada y almacenada.
El sistema digestivo es uno de los nueve sistemas principales del cuerpo humano y es el principal responsable de la ingesta, digestión, absorción y excreción de los alimentos. Consta de dos partes principales: el tracto digestivo y las glándulas digestivas.
El cáncer de pulmón es un tumor maligno que se origina en la mucosa bronquial o las glándulas de los pulmones. Es uno de los tumores malignos con mayor morbilidad y mortalidad y mayor amenaza para la salud y la vida humana.
La diabetes es una enfermedad crónica con hiperglucemia como signo principal. Es causada principalmente por una disminución en la secreción de insulina causada por una disfunción de las células de los islotes pancreáticos, o porque el cuerpo es insensible a la acción de la insulina (es decir, resistencia a la insulina), o ambas cosas. la glucosa en la sangre es ineficaz para ser utilizada y almacenada.
El sistema digestivo es uno de los nueve sistemas principales del cuerpo humano y es el principal responsable de la ingesta, digestión, absorción y excreción de los alimentos. Consta de dos partes principales: el tracto digestivo y las glándulas digestivas.
enzyme
The nature of enzymes
chemical nature
Most are proteins and a few are RNA
synthetic raw materials
Amino acids (proteins), ribonucleotides (RNA)
synthesis place
Ribosomes (proteins), nucleus (eukaryotes)
place of action
Inside the cell, outside the cell, outside the organism
source
Living cells
Physiological function
Has biocatalytic effect
An exploration of the nature of enzymes
The discovery of winemaking
Pasteur: Fermentation requires the participation of living yeast bacteria
debate <————>
Liebig: When yeast cells die, they release certain substances that cause fermentation.
Büchner: Obtained extracts from cells containing enzymes that caused fermentation
Speculate
Scientists speculate that enzymes are proteins
prove
Sumner: Obtained urease and proved that urease is a protein
Other scientists: Obtained crystals of pepsin, trypsin and many other enzymes and proved that these enzymes are proteins
Complements
Cech and Altman discover that a small number of RNAs also have biocatalytic functions
Enzyme properties
Efficiency
Ensure rapid cell metabolism
The efficiency of enzymes is relative to that of inorganic catalysts. Therefore, it must be compared with the catalytic effect of inorganic catalysts to prove that enzymes are highly efficient.
specificity
Each enzyme can only catalyze one or a type of chemical reaction to ensure that cell metabolism proceeds in an orderly manner.
The role of enzymes
Role: Enzymes have a catalytic role in cell metabolism (not regulation)
Mechanism of action: Reduce the activation energy of chemical reactions
Meaning: Make cell metabolism proceed quickly and orderly under mild conditions
Precautions
①Enzymes only have catalytic function and have no regulatory function ②The chemical properties and quantity of the enzyme remain unchanged before and after the reaction ③Enzymes can function both inside and outside cells ④ Under the catalysis of enzymes, cell metabolism proceeds rapidly under mild conditions ⑤Enzymes generally work by combining with specific substrates
Hydrogen peroxide decomposition experiment
Experimental reagents: fresh liver grinding fluid with a mass fraction of 20%, newly prepared hydrogen peroxide solution with a volume fraction of 3%, and FeCl3 solution with a mass fraction of 5%
Take four test tubes and add 2 mL of hydrogen peroxide solution respectively. Test tube No. 1 is left untreated. Test tube No. 2 is heated in a 90°C water bath. Test tube No. 3 is added with 2 drops of FeCl3 solution. Test tube No. 4 is added with 3 drops of liver grinding solution. There are basically no bubbles in the test tube. There are a few bubbles in test tube No. 2. There are more bubbles in test tube No. 3. There are a lot of bubbles in test tube No. 4. Put the lit sanitary incense into test tubes No. 3 and 4. Test tube No. 3 has a lot of bubbles. The re-ignition was not obvious, but the re-ignition in test tube No. 4 was obvious.
Experimental results
① Water bath heating, FeCl3 solution and catalase can all speed up the decomposition rate of hydrogen peroxide ② Compared with the inorganic catalyst FeCl3, the catalytic efficiency of catalase is much higher
Precautions
① Heating provides energy for the reaction and cannot reduce the activation energy. ②The essence of enzyme catalysis is to reduce the chemical energy in chemical reactions
Conditions that affect enzyme activity
temperature
experiment
test tube 1
Add 1mL amylase solution
Ice water bath for about 5 minutes
Mix well and then bathe in ice water for about 2 minutes
Take out, add two drops of iodine solution, shake, turn light blue
Test tube 1*
Add 2 mL of soluble amylase solution
Ice water bath for about 5 minutes
Mix well and then bathe in ice water for about 2 minutes
Take out, add two drops of iodine solution, shake, turn light blue
test tube 2
Add 1mL amylase solution
60℃ warm water bath for about 5 minutes
After mixing and shaking, place in a warm water bath at 60°C for about 2 minutes.
Take it out, add two drops of iodine solution, and shake until it does not turn blue.
Test tube 2*
Add 2 mL of soluble amylase solution
60℃ warm water bath for about 5 minutes
After mixing and shaking, place in a warm water bath at 60°C for about 2 minutes.
Take it out, add two drops of iodine solution, and shake until it does not turn blue.
test tube 3
Add 1mL amylase solution
Boiling water bath for about 5 minutes
Mix well and put in boiling water bath for about 2 minutes
Take it out, add two drops of iodine solution, shake, and turn blue.
Test tube 3*
Add 2 mL of soluble amylase solution
Boiling water bath for about 5 minutes
Mix well and put in boiling water bath for about 2 minutes
Take it out, add two drops of iodine solution, shake, and turn blue.
in conclusion
Within a certain range, as the temperature increases, the activity of the enzyme increases; beyond the optimal temperature, the activity of the enzyme gradually weakens or even loses. If the temperature is too high, the spatial structure of the enzyme will be destroyed and the enzyme will be permanently inactivated. However, low temperature only inhibits the activity of the enzyme. The spatial structure of the enzyme will recover as the temperature rises, so the enzyme is suitable to be stored at low temperature.
pH
experiment
Test tube number one
Two drops of fresh 20% liver grinding liquid
1mL pH=7 buffer
2mL of 3% hydrogen peroxide solution
A large number of bubbles are produced
Test tube number two
Two drops of fresh 20% liver grinding liquid
1mL 0.01mol\L hydrochloric acid solution
2mL of 3% hydrogen peroxide solution
Basically no bubbles produced
Test tube No. 3
Two drops of fresh 20% liver grinding liquid
1mL 0.01mol\L NaOH solution
2mL of 3% hydrogen peroxide solution
Basically no bubbles produced
in conclusion
Enzyme catalysis requires a suitable pH. High or low pH will affect the activity of the enzyme.