In 1810, Gaylussaac discovered that yeast can convert sugar into alcohol. This was an early germination of enzymology research.

In 1857, Pastuer believed that alcoholic fermentation was the result of yeast cell activity, Emphasis on the importance of living cells.

In 1876, William Kuhne proposed the word "Enzyme" to refer to various enzymes. This laid the foundation for the identical naming of enzymes.

In 1897, the Buchner brothers successfully achieved fermentation using cell-free yeast juice. This discovery opened the door to modern enzymology and biochemistry. Buchner also won the 1907 Nobel Prize in Chemistry.

In 1926, Sumner isolated and purified urease crystals from sword bean seeds. It was demonstrated for the first time that enzymes are proteins with catalytic activity.

1930-1936, Northrop obtained the crystals of pepsin and trypsin, It was further determined that the chemical nature of enzymes is proteins.

In 1982, Cech and Altman discovered that RNA has a catalytic effect in their research on Tetrahymena. The discovery of ribozymes broke the traditional concept that enzymes were proteins.

In 2007, Yan Xiyun's team discovered that ferric oxide nanoparticles have properties similar to horseradish peroxidase, thus proposing a new concept of nanozymes. This discovery is considered a landmark event in the history of enzymology. It blurs the boundaries between inorganic materials and organic organisms and promotes the integration of nanoscience and medicine.