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Lipidomics analysis methods for cell lines
This is a mind map about Lipidomics analysis methods for cell lines,Main content: Applications,Bioinformatics tools,Data analysis,Lipid detection,Lipid separation,Sample preparation.
Edited at 2025-01-20 08:30:50- Lipidomics analysis methods for cell lines
This is a mind map about Lipidomics analysis methods for cell lines,Main content: Applications,Bioinformatics tools,Data analysis,Lipid detection,Lipid separation,Sample preparation.
- Method for evaluating the proliferation ability of cell lines
This is a mind map about Method for evaluating the proliferation ability of cell lines,Main content: Limitations and considerations,Applications,Interpretation of results,Controls and standards,Data analysis,Imaging techniques,Proliferation assays,Experimental setup,Introduction.
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Lipidomics analysis methods for cell lines
- Lipidomics analysis methods for cell lines
This is a mind map about Lipidomics analysis methods for cell lines,Main content: Applications,Bioinformatics tools,Data analysis,Lipid detection,Lipid separation,Sample preparation.
- Method for evaluating the proliferation ability of cell lines
This is a mind map about Method for evaluating the proliferation ability of cell lines,Main content: Limitations and considerations,Applications,Interpretation of results,Controls and standards,Data analysis,Imaging techniques,Proliferation assays,Experimental setup,Introduction.
- Plant Introduction to Cucumber
This is a mind map about Plant Introduction to Cucumber,Main content: Cucumber Recipes,Varieties and Cultivars,Harvesting and Storage,Growing Cucumbers,Culinary Uses,Health Benefits,Nutritional Value,Cucumber Overview.
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Lipidomics analysis methods for cell lines
Sample preparation
Cell lysis
Mechanical disruption
Use of sonication
Use of French press
Chemical lysis
Use of detergents
Use of organic solvents
Protein precipitation
Addition of methanol or acetonitrile
Centrifugation to remove precipitated proteins
Extraction of lipids
Folch method (chloroform/methanol)
Bligh and Dyer method (chloroform/methanol/water)
Lipid separation
Thin-layer chromatography (TLC)
Silica gel plates
Visualization with iodine or specific stains
High-performance liquid chromatography (HPLC)
Normal phase
Separation based on polarity
Reverse phase
Separation based on hydrophobicity
Gas chromatography (GC)
Derivatization of lipids
Formation of volatile derivatives
Separation based on volatility and boiling point
Lipid detection
Mass spectrometry (MS)
Electrospray ionization (ESI)
Soft ionization technique
Matrix-assisted laser desorption/ionization (MALDI)
Use of matrix to assist in ionization
Tandem MS (MS/MS)
Fragmentation of lipids for structural information
Nuclear magnetic resonance (NMR) spectroscopy
Identification of lipid classes and fatty acid composition
Quantification of lipids
Data analysis
Peak identification
Use of databases (e.g., Lipid Maps, HMDB)
Comparison with standards
Quantification
Internal standards
Addition of known amounts of isotopically labeled lipids
External standards
Calibration curves for quantification
Statistical analysis
Multivariate analysis
Principal component analysis (PCA)
Partial least squares discriminant analysis (PLS-DA)
Univariate analysis
ANOVA
ttest
Bioinformatics tools
Lipid identification software
LipidSearch
LipidBlast
Data normalization
Normalization to total lipid content
Normalization to cell number or protein content
Pathway analysis
Integration with genomic and proteomic data
Metabolic pathway mapping
Applications
Disease biomarker discovery
Identification of lipid changes in disease states
Correlation with disease progression
Drug discovery
Assessment of drug effects on lipid metabolism
Identification of potential drug targets
Systems biology
Understanding lipid metabolism in cellular systems
Modeling lipid networks and their interactions